cloning small DNA molecules

j.c.m.oosterman j.c.m.oosterman at inter.nl.net
Mon Aug 28 14:42:03 EST 2000


Hi all,

Is there anyone having experience with the cloning of small (20-30bp) DNA
fragments? Can agarose (are there special types, what concentration?) still
be used for separation of such small DNA fragments (rather than
polyacrylamide for which I have to buy new electophoresis units). Do small
DNA fragments preciptate as efficient as large ones using ethanol
precipitation or are there other ways to purify small DNA fragment.

Thank for helping me,
Jan







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