G418 resistance gene
rosie.redfield at zoology.oxford.ac.uk
Mon Aug 28 15:03:32 EST 2000
I'm trying to develop a transformation system for Chilomonas, a eukaryotic
alga. The cells are very sensitive to G418, so I'm looking for a vector
with a neo resistance gene under a suitable promoter.
The problem is, we know nothing about Chilomonas promoters. The slow and
sure approach will be to clone a Chilomonas tubulin gene and construct a
neoR vector using its promoter, but until that's done I'd like to try
transfroming the cells with a neoR gene under a heterologous promoter. A
plant promoter would be worth a try, though of course an algal promoter, if
one is available, would be even better.
Does anyone know of any vectors where the neoR gene is driven by an algal
or plant promoter?
Thanks very much.
Dr. Rosemary J. Redfield redfield at interchange.ubc.ca | either email
rosie.redfield at zoo.ox.ac.uk | will work fine.
On sabbatical until July 2001 at:
Dept. of Zoology, University of Oxford, Oxford UK OX1 3PS
lab phone (0)1865 281306; mobile phone (0)7730 368570
Associate Professor Department of Zoology
Univ. of British Columbia Office: (604) 822-3744
Vancouver, B.C. V6T 1Z4 Lab: (604) 822-6323
Canada Fax: (604) 822-2416
WWW site: http://www.zoology.ubc.ca/~redfield
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