dNTP problem

[Tom Knight]

andreip at byte.cas.vanderbilt.edu (Andrei Popescu) writes:

> So it seems to depend on whether the dNTPs are
> premixed or mixed by me. Either I am doing something systematically
> wrong when mixing , or there is something more subtle.

What are you mixing it with?  An obvious question is what your local
water quality looks like.  Try commercial nuclease free water or
borrow some water from the lab that your experiment works in.