cloning small DNA molecules

Ned Mantei mantei at cell.biol.ethz.ch
Tue Aug 29 01:27:55 EST 2000


In article <8oef78$qev$1 at porthos.nl.uu.net>, "j.c.m.oosterman" 
<j.c.m.oosterman at inter.nl.net> wrote:

>Hi all,
>
>Is there anyone having experience with the cloning of small (20-30bp) DNA
>fragments?

If you know the sequence, the easiest thing is to have a double-stranded 
oligonucleotide synthesized. Kinase the ends or have the oligos 
synthesized with 5'-phosphates.

-- 
Ned Mantei
Department of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland






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