cloning small DNA molecules
mantei at cell.biol.ethz.ch
Tue Aug 29 01:27:55 EST 2000
In article <8oef78$qev$1 at porthos.nl.uu.net>, "j.c.m.oosterman"
<j.c.m.oosterman at inter.nl.net> wrote:
>Is there anyone having experience with the cloning of small (20-30bp) DNA
If you know the sequence, the easiest thing is to have a double-stranded
oligonucleotide synthesized. Kinase the ends or have the oligos
synthesized with 5'-phosphates.
Department of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
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