cloning small DNA molecules

Ned Mantei mantei at
Tue Aug 29 01:27:55 EST 2000

In article <8oef78$qev$1 at>, "j.c.m.oosterman" 
<j.c.m.oosterman at> wrote:

>Hi all,
>Is there anyone having experience with the cloning of small (20-30bp) DNA

If you know the sequence, the easiest thing is to have a double-stranded 
oligonucleotide synthesized. Kinase the ends or have the oligos 
synthesized with 5'-phosphates.

Ned Mantei
Department of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland

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