dNTP problem

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Aug 29 03:32:03 EST 2000


In article <mantei-B47484.08313729082000 at news.ethz.ch>, Ned Mantei
<mantei at cell.biol.ethz.ch> writes
>In article <39AB188F.608D3A57 at med.monash.edu.au>, Paul Cullen 
><Paul.Cullen at med.monash.edu.au> wrote:
>
>>Make sure you are not defrosting them in a water bath. dNTPs are quite
>>sensitive to heat and should be stored at -20 C. Defrost on ice.
>
>Although I generally agree that keeping reagents cold is a good idea, 
>dNTPs can't be as sensitive as all that. In the course of a PCR they 
>survive maybe an hour or more at 72 C and half an hour to an hour at 94 
>C.

They can also be shipped at RT.

>Incidentally, I have heard the claim that MgCl2 doesn't redissolve 
>easily and needs to be warmed after thawing.

Never come across that one in many years of producing PCR reagents.

Duncan

-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






More information about the Methods mailing list