Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Tue Aug 29 03:32:03 EST 2000
In article <mantei-B47484.08313729082000 at news.ethz.ch>, Ned Mantei
<mantei at cell.biol.ethz.ch> writes
>In article <39AB188F.608D3A57 at med.monash.edu.au>, Paul Cullen
><Paul.Cullen at med.monash.edu.au> wrote:
>>Make sure you are not defrosting them in a water bath. dNTPs are quite
>>sensitive to heat and should be stored at -20 C. Defrost on ice.
>Although I generally agree that keeping reagents cold is a good idea,
>dNTPs can't be as sensitive as all that. In the course of a PCR they
>survive maybe an hour or more at 72 C and half an hour to an hour at 94
They can also be shipped at RT.
>Incidentally, I have heard the claim that MgCl2 doesn't redissolve
>easily and needs to be warmed after thawing.
Never come across that one in many years of producing PCR reagents.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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