dNTP problem

Chris LaRosa clarosa at biocomp.unl.edu
Tue Aug 29 08:21:53 EST 2000



Ned Mantei wrote:
> 
> In article <39AB188F.608D3A57 at med.monash.edu.au>, Paul Cullen
> <Paul.Cullen at med.monash.edu.au> wrote:
> 
> >Make sure you are not defrosting them in a water bath. dNTPs are quite
> >sensitive to heat and should be stored at -20 C. Defrost on ice.
> 
>

hmm I am not sure they are that sensitive to heat as I always put them
in the 37 degree water bath.   So far my reactions keep working.  
The comment about the water quality might be a good bet.  Another often
overlooked but critical "reagent".

You might have the other lab prepare you dntps....






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