native agarose electrophoresis of megadalton protein complexes

A. J. Bowling abowling at
Tue Aug 29 10:34:02 EST 2000

has anyone out there had any experience and/or luck separating large 
(megadalton) native protein complexes on agarose gels?  in fact, any info on 
electrophoresing proteins in agarose gels would be useful (ie TAE buffer or 
normal acrylamide buffers, or something else?)  im having trouble getting the 
coomassie out of the gel after staining.  any ideas about this?  

thanks in advance!

andrew bowling
university of texas at austin

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