need help for the transformation

[Chris LaRosa]

> 
> Any suggestion is wellcome, thank you in advance.
> 
> Bo
> 
You might do minipreps from your single colonies , cut with restriction
enzymes bordering the insert and check for the number of inserts.  If
you get too many co-transformation events, you may have to do a series
of transformations varying the ratio of cells to ligation mixture and
empirically find the ligation mix that gives a high proportion of single
plasmid transformants.   I suppose you could rescue your library by
purifying the plasmids and retransforming in a series as outlined
above.