need help for the transformation

Chris LaRosa clarosa at biocomp.unl.edu
Tue Aug 29 11:05:06 EST 2000


> 
> Any suggestion is wellcome, thank you in advance.
> 
> Bo
> 
You might do minipreps from your single colonies , cut with restriction
enzymes bordering the insert and check for the number of inserts.  If
you get too many co-transformation events, you may have to do a series
of transformations varying the ratio of cells to ligation mixture and
empirically find the ligation mix that gives a high proportion of single
plasmid transformants.   I suppose you could rescue your library by
purifying the plasmids and retransforming in a series as outlined
above.






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