How to bring as much DNA as possible into the well?

R. John Lye rjl6n at virginia.edu
Tue Aug 29 13:16:26 EST 2000


bo pang wrote:

> Even though I tried my best to do
> the loading job, some of the sample still float out of the wells. I also
> tried to use a higher percentage of loading dye for the sample mixture,
> but it didn't help either.

Are you rinsing the excess urea out of the well *immediately* before
loading the gel?  Some urea will diffuse out of the gel into the sample
well, preventing your sample from sinking to the bottom if you don't
rinse out the wells right before you load them.

John









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