need help for the transformation

Chris LaRosa clarosa at biocomp.unl.edu
Tue Aug 29 15:25:22 EST 2000


> I would think this is unlikely. Usually the incompatibility regions of
> the plasmid will stop that occurring.
> 
> 
Incompatability regions..?  hmm please elaborate.. I know that
co-transformation is possible in plant and dicty cells... What about E.
coli?  I was assuming that the poster did get clean single clone ,
single colonies, but it seems that this might be a reason for unreadable
insert sequence.






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