mathieu.ingouff at sgen.slu.se
Thu Aug 31 04:22:11 EST 2000
I am amplifying my gene by PCR using cDNAs.
I do the RT with a d17-Adapter and then amplify with one specific primer
and the adapter primer.
And i get a single band that would correspond to a truncated amplified
cDNA. The expected size if 3 kb and i get a 2kb band.
I tried again using DNA prepared from a cDNA library (RT was done with a
Actually i don't know if it is truncated but my gene belong to a highly
conserved family and this protein would miss one conserved domain but
well i cannot get it......
DO you have any idea how i can really be sure that i have the full
I believed that if the d17 would bind in several places i would see
several bands but i don't.
Thank you for your help
Mathieu Ingouff, PhD student
SLU, Department of Forest Genetics
Genetikvagen 1B, Box 7027
S-750 07 Uppsala, Sweden
email: mathieu.ingouff at sgen.slu.se
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