Truncated cDNA?

[Mathieu Ingouff]

Hello,

I am amplifying my gene by PCR using cDNAs.
I do the RT with a d17-Adapter and then amplify with one specific primer
and the adapter primer.
And i get a single band that would correspond to a truncated amplified
cDNA. The expected size if 3 kb and i get a 2kb band.
I tried again using DNA prepared from a cDNA library (RT was done with a
d17 primer)
Same result.

Actually i don't know if it is truncated but my gene belong to a highly
conserved family and this protein would miss one conserved domain but
well i cannot get it......

DO you have any idea how i can really be sure that i have the full
length?
I believed that if the d17 would bind in several places i would see
several bands but i don't.

Thank you for your help

Mathieu
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____________________________________
  Mathieu Ingouff, PhD student
  SLU, Department of  Forest Genetics
  Genetikvagen 1B, Box 7027
  S-750 07 Uppsala, Sweden

  email: mathieu.ingouff at sgen.slu.se
  fax: +46.18.67.32.79.
 phone: +46.18.67.32.32.
____________________________________



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