Growth-rate determination by phosphatase assay

Kresten kresten at my-deja.com
Thu Aug 31 06:55:29 EST 2000


Does anybody have experience with the Acid Phosphatase method for
quantifying growth of cell cultures (e.g. mammalian cells). It is
described in:
Yang, Sinai and Kain
Anal Biochem 241: 103-108, 1996

Any problems, tricks etc. What numbers of cells pr. well (96 well
plate) have been measured successfully?

thank you
Kresten




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