DH5a vs XL1Blue
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Fri Dec 1 08:53:20 EST 2000
In article <90810a$8fb$1 at wnnews.sci.kun.nl>, the eminent Rudi van de
Wetering at University of Nijmegen, The Netherlands wrote
>Thougth you also had to add Maltose for selection/expression of the F' (at
>least if you use LB).
Maltose will have no effect on selection of the F'. What maltose will do
is repress lac expression even more. The F' in XL1Blue is Tetracycline
resistant so selection with Tet is all one needs to maintain the F'.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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