Lambda digested with PstI

Arnoud van Vliet avvliet at knoware.nl
Fri Dec 8 17:44:20 EST 2000


> you are sure it was PstI?? It has 28 sites within the lambda genome.
> therefore it's map is not listed in the catalogues.
> HAve a look in the New England Biolabs or the MBI Fermentas catalogues
> to find a better enzyme for digesting Lambda (e.g. HindIII)

Lambda x PstI is a fine size marker, although the 1 kb style markers are
easier. I have used the lambda x PstI marker during my Ph.D. project, and
passed. So it can't be that bad :)

Making lambda x PstI is easy, and cheaper than the commercially available
markers

Arnoud








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