Taqman 'VIC' dye proper compound name.

tfitzwater at gilead.com tfitzwater at gilead.com
Mon Dec 11 21:23:34 EST 2000


>If someone has a good list of the most used dyes, compound names and
>absorbance emissions wavelengths I would love a copy.


>It seems a tad hard to get hold of what the VIC really is.. Tm I
>suppose. >Tnx, Moje



Wavelengths for Taqman Dyes (information assembled from these references:
Dan Shaffer <dshaffer at jax.org> via 7700taqman at listserv.it.northwestern.edu
RE: VIC emission wavelength maxima, ABI at
http://pebiopcr-sds.custhelp.com/cgi-bin/pcr/faq, Molecular Probes at
http://www.probes.com/handbook/ and Glen Research at
http://www.glenres.com/)

DYE/Excitation Wavelength (nm)/Emission Wavelength (nm)/Extinction
Coefficient (M-1cm-1)
FAM (reporter)/488/518/20,958 at 260
TET (reporter)/     488/538/16,255 at 260
HEX (reporter)/488/556/31,580 at 260
JOE (reporter)/     488/554/12,000 at 260
VIC (reporter)/     488/552/30,100 at 260
TAMRA (quencher)/488/582/31,980 at 260
SYBR (reporter)/488/520/NA
ROX (passive reference)/488/~610/NA
NFQ (non-fluorescent Quencher)/NA/NA/79,300 at 260

5-FAM: amine-reactive succinimidyl ester of carboxyfluorescein,
5-carboxyfluorescein, succinimidyl ester (5-FAM, SE)
TET: 5'-Tetrachloro-Fluorescein
HEX: 5'-Hexachloro-Fluorescein
6-JOE: 6-carboxy-4',5'-dichloro-2', 7'-dimethoxyfluorescein, succinimidyl
ester (6-JOE, SE)
VIC: proprietary, no data found on Applera/Applied Biosystems website.
6-TAMRA: 6-carboxytetramethylrhodamine, succinimidyl ester (6-TAMRA, SE)
SYBR: proprietary, no data found on Molecular Probes website.
6-ROX: 6-carboxy-X-rhodamine, succinimidyl ester (6-ROX, SE)

Tim Fitzwater
Principal Research Associate
Gilead Sciences



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