The People's Seed Bank

Brian Sandle bsandle at caverock.net.nz
Tue Dec 12 00:12:28 EST 2000


On Tue, 12 Dec 2000, David Kendra wrote:

> 
> "Brian Sandle" <bsandle at caverock.net.nz> wrote in message
> :  On Mon, 11 Dec 2000, David
> : Kendra wrote:
> :
> : > I think folks do that in demo classes for the gun.  I used to do it for
> : > demonstrations when we had folks tour our facility.  What do you think
> the
> : > result was?
> :
> : that the genes themselves were a factor because as
> : you wrote in reply to Tracy:
> :
> : >The level of somaclonal variation depends on the genetic background of
> : the
> : >line used in the transformation.  In maize some lines exhibit strong
> : >somaclonal variation while others do not.
> 
> The genetic background influences the level of somaclonal variation, not the
> gene being introduced by the biolistic transformation process.

You are trying to say that the introduced gene has minimal effect on the
genetic background, but the process can change the compostion of the plant
considerably.

Besides where is your evidence?
 :
> : I do not believe that the strong promoters are under the normal control of
> : the plant.
> 
> So you do not believe strong promoters can be silenced by a plants gene
> silencing system?

Not easily, according to Mae-Wan Ho.

[Reading to do].






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