Taqman 'VIC' dye proper compound name.
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Tue Dec 12 04:57:48 EST 2000
In article <90nksm$1p$1 at nnrp1.deja.com>, the eminent at Deja.com -
Before you buy. wrote
>If someone has a good list of the most used dyes, compound names and
>absorbance emissions wavelenghts I would love a copy.
Dye Excitation Emission
Fluorescein 494 525
6-FAM 492 515
TET 521 536
JOE 527 548
HEX 535 556
TAMRA 555 580
Cy3 552 565
ROX 568 595
6-ROX 575 602
Texas Red 583 603
Rhodamine 6G 518 543
Sybr Green I 495 520
Sybr Gold 300/495 537
I've pulled these from a Eurogentec Spring handout so I hope they are
right! Sybr from Mol. Probes
Details on the ABI Taqman website are a bit misleading in that the 7700
has a 488 laser plus a secondary excitation line taken off that (prism?)
for the higher excitation dyes i.e. JOE
Their ROX passive reference is a FRET oligo so don't be misled by their
excitation at 488 and emission at around 600.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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