shearing of DNA

Ian A. York iayork at panix.com
Thu Dec 14 20:40:26 EST 2000


In article <3A396972.F4422508 at u.washington.edu>,
Andreas Schramm  <schramm at u.washington.edu> wrote:
>
>I'm currently looking for a protocol to (randomly!) shear DNA to get
>fragments of
>1-2 kb length.

Have a look at 
<http://www.bio.net//hypermail/METHDS-REAGNTS/methods.199705/0273.html>.

I used a nebulizer to shear my DNA; it worked very nicely.  Doing a web 
search of "shearing DNA nebulizer" will get you some fairly detailed 
protocols.

Ian 
-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England






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