[Q]dissociation in high salts

Kim, Chun gidarim at family.sogang.ac.kr
Tue Dec 19 10:24:44 EST 2000


I'm purifying a protein complex from fly extract. It binds well 
to DEAE-sepharose and is eluted w/ 1.5M KOAcetate, but not in 
lower salt conditions. Binding and elution wer confirmed by western 
using a specific antibody for a component in the complex. What I'm 
concerned is wheather the components of the complex are dissociated 
in such a high salt condition.

any suggestion will be helpful.

     The reasonable man adapts himself to the world; the unreasonable
     one persists in trying to adapt the world to himself. Therefore,
     all progress depends on the unreasonable man.

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