[Q]dissociation in high salts
gidarim at family.sogang.ac.kr
Tue Dec 19 10:24:44 EST 2000
I'm purifying a protein complex from fly extract. It binds well
to DEAE-sepharose and is eluted w/ 1.5M KOAcetate, but not in
lower salt conditions. Binding and elution wer confirmed by western
using a specific antibody for a component in the complex. What I'm
concerned is wheather the components of the complex are dissociated
in such a high salt condition.
any suggestion will be helpful.
The reasonable man adapts himself to the world; the unreasonable
one persists in trying to adapt the world to himself. Therefore,
all progress depends on the unreasonable man.
More information about the Methods