Does Nitrocellulose bind single strand DNA?

Alejandro Miguel Martin Dunn alejandro.martin at cigb.edu.cu
Wed Dec 27 09:56:37 EST 2000


Yes it does (that's what most people used before nylon-based filters
were commercially available). Just make sure you bind in high ionic
strength.

cheers,
alejandro

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Alejandro Martin
Centro de Ingenieria Genetica y Biotecnologia
PO Box 6162, C. Postal 10600
Ciudad Habana, CUBA
Tel: (53-7)216221, FAX: (53-7)214764

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>-----Original Message-----
>From: Stephen Snowdy [mailto:snowds at med.unc.edu]
>Sent: Thursday, December 21, 2000 4:15 PM
>To: methods at hgmp.mrc.ac.uk
>Subject: Does Nitrocellulose bind single strand DNA?
>
>
>I know that it doesn't bind double stranded, but I'm not sure about SS.
>Thanks for your time.
>-- 
>Stephen Snowdy
>
>
>


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