RNA Denaturation on PAGE

Choona xuna68 at hotmail.com
Tue Feb 1 12:46:09 EST 2000


Hi,

Should polyacrylamide gels containing 7M urea be run at elevated temp to
ensure denaturating conditions throughout? Should the gel be pre-heated? I
heat my samples in formamide buffer to 70, but worry my RNA is denaturing
whilst running.
Please reply to cb252 at mole.bio.cam.ac.uk as I've borrowed a friend's
account.
Thanks so so much!!!!
Claire Balding






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