R: Kozak consensus question

Monod bagnaresi at libero.it
Tue Feb 1 15:37:26 EST 2000


if you hold that the minimum Kozak's consensus is considered to be
 (A/G)XX AUG G
where X is any base,  looks like your option a) doesn't keep the consensus,
while option b) should be fine..
I'm use in fact  to consider strictly necessary the A/G at -3 and the G at
+4
also, the Kozak's consensus has more recently been refined for some
organisms (e.g, for plants, you can find it in Plant Mol Biol 35: 993-1001)
so
you may find some more uptaded consensus (perhaps..)


"Wolfgang Schechinger" <Wolfgang.Schechinger at med.uni-tuebingen.de> wrote in
message 200001311427.PAA13693 at intwww.zit.med.uni-tuebingen.de...
> Hi all,
> I have a squence like this: GCC GCC ACC ATG G; it contains a Kozak
> consus element in order to ensure efficient translation.
>
> I'd like to introduce a NdeI restriction site (catatg).
>
> What might be the better solution:
> a) mutating the sequnce to GCC GCC cat ATG G (mutations are lower
> case)
>
> b) inserting two bases; the sequence then would be GCC GCC ACC at ATG
> G
>
> will either of the two ways destroy the effect of the Kozak element?
>
> Thaks for all input!
>
> Wolfgang
>
> Dr. Wolfgang Schechinger, Pathobiochemistry Dept.
> University of Tuebingen, Germany
> email: wolfgang.schechinger at med.uni-tuebingen.de
> wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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