hairpins in PCR primers

alex dobrovic adobrovic at medicine.adelaide.edu.au
Tue Feb 1 23:49:27 EST 2000


Does anyone know if hairpins in PCR primers really affect PCR
amplifications? Most PCR reactions would be carried out at a temperature
far in excess of the melting temperature of the hairpin.

Regards

Alex


adobrovic at medicine.adelaide.edu.au


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