35S-labelled oligo purification

Danny McLaughlin D.P.McLaughlin at mds.qmw.ac.uk
Wed Feb 2 09:09:34 EST 2000


Duncan,

> Am I missing something here? How are you labelling oligos with 35S dATP?
> Are you not kinasing using T4PNK and gamma labelled 35S ATP?

Nope, 3' end labelling with terminal transferase.  5'  tailing doesn't give
a hot enough probe for in situ hybridzation.

Danny


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Dr. Danny McLaughlin
Lecturer in Neuropharmacology
Anaesthetics Unit
Division of Surgery, Clinical Neuroscience & Intensive Care
St. Bartholomew's and The Royal London School of Medicine & Dentistry
The Royal London Hospital
Whitechapel
London E1 1BB

Tel:   +44 (0)207 377-7725 or 377-7000 ext 2292 (answerphone)
Fax:  +44 (0)207 377-7126

Email:  d.p.mclaughlin at mds.qmw.ac.uk
If mail is bouncing, try dmclaugh at hgmp.mrc.ac.uk
URL:  http://www.mds.qmw.ac.uk/anae/neurolab.htm
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