method for transcription start determination

soenke behrends behrends at plexus.uke.uni-hamburg.de
Wed Feb 2 11:57:21 EST 2000



Dear netters,

what is the easiest and most straightforward
method of transcription start analysis. We
have isolated cDNA and 2000 bp of 5 prime 
flanking genomic DNA with potential TATA
Signals. 


Primer extension analysis?
RNase protection analysis?
Or has anyone tried a simple RT-PCR approach
using different primer pairs to map the 
transcription start?

Thanks for your time and help
Soenke Behrends








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