electroporation

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Wed Feb 2 17:26:32 EST 2000


:Hi everybody,
:
:Other than BioRad, is there any other company that makes electroporation 
:machines?  Do you recommend them?
:

BTX is the most reasonable alternative to Bio-Rad. IMO, all this is 
insanely overpriced. Invitrogene used to sell (not anymore) the most 
simple and cheapest electroporators available - basically a cuvette 
holder, electronic switch, series of capacitors (25 - 990 uF - the later 
being three 330 uF x 250V in parallel), and a series of resistances 50 - 
10,000 Ohms in parallel to the sample. The whole thing was to be 
plugged to the regular power supply found in every lab. Any qualified 
electronic shop can build this for $100-150 parts and a day of making.

        - Dima






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