Introns in monocot constructs

Joe Petrash petras at
Thu Feb 3 00:37:36 EST 2000

I'm in the middle of preparing a monocot promoter for tissue-specific
expression, intending to use the promoter to drive GUS (barley
transformation). The native barley gene has no intron in either 5' or
coding region. Does anyone know how likely I am to need to add an intron
(e.g.ubiquitin or actin) for stable or high levels of expression, and if
so, how great is the risk of losing the tissue specific and inducible


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