Low RNA yield

Danny McLaughlin D.P.McLaughlin at mds.qmw.ac.uk
Thu Feb 3 04:44:28 EST 2000


> She says that she is getting low yields of RNA from thymus cell
> culture.  She uses Qiagen RNAeasy kit.  The cell number in her samples
> is 1 x 10(7), which is the max starting amount recommended.

I think this may be the root of the problem - if the columns get overloaded,
they are prone to fail, just like plasmid extractions done from superbroths
like 2 X YT.  Has your friend thought of using fewer cultured cells??

Danny








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