Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Thu Feb 3 04:24:46 EST 2000
In article <3897feb2 at redeye.it.ki.se>, Csaba Kiss <csakis at ki.se> writes
>When I looked at several papers I saw that when they used an oligoT primer
>to capture polyA RNA, the sequence looked like this T(30)N(2). Is it because
>of the synthesizing of the primer is better this way, or it improves the
>annealing properties of the primer?
Possibly T(30)VN? V is anything but T so in thery the oligo latches onto
the join between the end of the coding sequence and the polyA rather
than anywhere on the polyA. Cuts down on cloning or sequencing or RT-
PCRing several hundred A's.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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