Medium without phosphate

Rafael Maldonado rmaldonado at ua.es
Thu Feb 3 09:56:39 EST 2000



ChenHA wrote:
> 
> Pardon my ignorance, but can you really get a minimal medium without
> phosphate?

I am sorry, I meant mininal or limiting amount of phosphate, as for
alkaline phosphatase induction. I need the cells in phosphate starvation
state.

> I should imagine it is an essential component (where else would
> the cells find the phosphate for its DNA, ATP etc.?).  There is the MOPS
> buffer (Neidhardt et al, (74), Journal of Bacteriology 119(3), 736-747)
> which works better than M9, but even that has a small amount of phosphate.

My library does not have JB that old. Would you mind to send me the
recipe? Thanks,

-Rafa




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