Medium without phosphate

ChenHA hzhen at freeuk.com
Thu Feb 3 10:47:19 EST 2000


ChenHA wrote:

>
> This is a rather long recipe:
>
> K2HPO4   0.132 mM
> MOPS      40 mM
> Tricine        4  mM
> NH4Cl       9.52 mM
> NaCl          50  mM
> Glucose        2 g
> MgSO4      0.5 mM
> CaCl2        0.5 uM
> FeSO4      0.01 mM  (make fresh)
> K2SO4     0.276 mM
>
> Trace elements (1000X concentration) - add 1 ml:
> ZnSO4    10 uM
> CoCl2      30 uM
> Amm. molybdate  3 uM
> CuSO4   10 uM
> H3BO3  400 uM
> MnSO4  80 uM

Sorry, I forgot to mentioned that it should be pH to 7.4 with KOH

>
> The medium should be filtered sterilised.  I think you can prepared the MOPS
> medium in concentrated form and stored, but you would need the paper to check it
> (I'm afraid I don't have it with me).  It may be useful to try and get the paper
> anyway as it has useful information about the constituents of this recipe.





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