freezing protein

Nick Theodorakis nicholas_theodorakis at
Thu Feb 3 11:34:54 EST 2000

In article <3899A465.F47628EA at>, mb <
mb at> wrote:
>Can someone point me to a good reference on determining optimal
>freezing conditions for purified protein?  I am interested in
the use
>of various additives and buffer systems to avoid precipitation
>oxidation.  Neither Current Protocols in Protein Science or
>Biology have anything on this.
>Mark Bowen

When my wife was making unstable chemical derivatives of a pure
protein, she would freeze the protein in small droplets by
dripping it with a pipette directly into liquid nitrogen. The
would freeze instantly into little balls of about 20-50 ul each.
Then store them in vials under liquid nitrogen; when you need
some, shake out the number of balls you need and you don't need
refreeze any.

Nick Theodorakis

nicholas_theodorakis at

* Sent from RemarQ The Internet's Discussion Network *
The fastest and easiest way to search and participate in Usenet - Free!

More information about the Methods mailing list