Brdu staining

[Nicolas Lemee]

We use RQ1 Dnase 1 from Promega.
--
Nicolas

dror_seliktar at my-deja.com wrote:

> Hi:
>
> I am trying to stain a monolayer of endothelial cells on a collagen
> lattice with anti-Brdu monoclonal antibody.  The assay requires a 20
> minute incubation with HCL (or microwave) to partially denature the
> DNA.  The denaturing step results in a complete destruction of the
> collagen substrate and subsequent loose of Endothelial cells.  I was
> wondering if there are methods for partial DNA denaturing which are not
> destructive to reconstituted collagen.
>
> Thank you,
>
> Dror Seliktar
> Georgia Institute of Technology
>
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