Brdu staining

Nicolas Lemee Lemee at attglobal.net
Thu Feb 3 12:44:24 EST 2000


We use RQ1 Dnase 1 from Promega.
--
Nicolas

dror_seliktar at my-deja.com wrote:

> Hi:
>
> I am trying to stain a monolayer of endothelial cells on a collagen
> lattice with anti-Brdu monoclonal antibody.  The assay requires a 20
> minute incubation with HCL (or microwave) to partially denature the
> DNA.  The denaturing step results in a complete destruction of the
> collagen substrate and subsequent loose of Endothelial cells.  I was
> wondering if there are methods for partial DNA denaturing which are not
> destructive to reconstituted collagen.
>
> Thank you,
>
> Dror Seliktar
> Georgia Institute of Technology
>
> Sent via Deja.com http://www.deja.com/
> Before you buy.





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