Lemee at attglobal.net
Thu Feb 3 12:44:24 EST 2000
We use RQ1 Dnase 1 from Promega.
dror_seliktar at my-deja.com wrote:
> I am trying to stain a monolayer of endothelial cells on a collagen
> lattice with anti-Brdu monoclonal antibody. The assay requires a 20
> minute incubation with HCL (or microwave) to partially denature the
> DNA. The denaturing step results in a complete destruction of the
> collagen substrate and subsequent loose of Endothelial cells. I was
> wondering if there are methods for partial DNA denaturing which are not
> destructive to reconstituted collagen.
> Thank you,
> Dror Seliktar
> Georgia Institute of Technology
> Sent via Deja.com http://www.deja.com/
> Before you buy.
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