freezing protein

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Thu Feb 3 13:07:45 EST 2000


:Can someone point me to a good reference on determining optimal
:freezing conditions for purified protein?  I am interested in the use
:of various additives and buffer systems to avoid precipitation and
:oxidation.  Neither Current Protocols in Protein Science or Molecular
:Biology have anything on this.

Or, alternatively, don't freeze :-)

Mix with equal volume of ethylene glycol in the presense of 2 mM 
DTT and store at -20C. 

        - Dima




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