transfection of oligos

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Thu Feb 3 15:06:23 EST 2000


In article <200002031854.TAA06710 at intwww.zit.med.uni-tuebingen.de>, 
Wolfgang.Schechinger at med.uni-tuebingen.de ("Wolfgang Schechinger") wrote:
:Scott, have a look there: http://www.liv.ac.uk/~giles/
:
:They use stroptolysine O and claim to have a high efficieny.
:

IMHO: whenever you see the word SLO, think "irreproducible". 

        - Dima

:> :Can anyone recommend a transfection agent with high efficiency for
:> the :introduction of oligonucleotides (anti-sense or molecular
:> beacons) into :generic cell lines like COS or CHO. :
:>  Electroporator would the best agent in this case.
:> 
:>         - Dima
:
:Dr. Wolfgang Schechinger, Pathobiochemistry Dept.      
:University of Tuebingen, Germany
:email: wolfgang.schechinger at med.uni-tuebingen.de 
:wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
:-----
:*unsolicited mail is *NOT* appreciated, usual disclaimers apply 
:-----                         
:Pressing ALT+F4 simultaneously enables you to read private messages
:---




More information about the Methods mailing list