transfection of oligos

flowman deszo at uiuc.edu
Fri Feb 4 09:59:34 EST 2000


I have seen good results with a commercial lipid-like product FuGENE6 etc...

Eric
"Dima Klenchin" <klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu> wrote in
message news:87cn5t$19fo$2 at news.doit.wisc.edu...
> In article <200002031854.TAA06710 at intwww.zit.med.uni-tuebingen.de>,
> Wolfgang.Schechinger at med.uni-tuebingen.de ("Wolfgang Schechinger") wrote:
> :Scott, have a look there: http://www.liv.ac.uk/~giles/
> :
> :They use stroptolysine O and claim to have a high efficieny.
> :
>
> IMHO: whenever you see the word SLO, think "irreproducible".
>
>         - Dima
>
> :> :Can anyone recommend a transfection agent with high efficiency for
> :> the :introduction of oligonucleotides (anti-sense or molecular
> :> beacons) into :generic cell lines like COS or CHO. :
> :>  Electroporator would the best agent in this case.
> :>
> :>         - Dima
> :
> :Dr. Wolfgang Schechinger, Pathobiochemistry Dept.
> :University of Tuebingen, Germany
> :email: wolfgang.schechinger at med.uni-tuebingen.de
> :wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
> :-----
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> :-----
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