genomic DNA from plants?
Jose I. de las Heras
Jose.delasHeras at hgu.mrc.ac.uk
Fri Feb 4 11:14:44 EST 2000
> I´m working with A. thaliana and have to do a lot of DNA minipreps with
> just two leaves (about 50-100 mg) of material. Can anybody recommend me
> a cheap (i. e. not based on commercial columns) and reliable prep that
> produces DNA which can be digested?
> Harald F. Sigmund
> hsigmund at edv2.boku.ac.at
Have you tried the standard CTAB-extraction method? I have used it
extensively for eucalypts and wheat. I usually extracted DNA from about
1-2g leaf tissue, to obtain a lot of DNA. But you can always scale it
down and modify it to make it quite fast too... Any questions email me.
Jose I. de las Heras Email: Jose.delasHeras at hgu.mrc.ac.uk
MRC Human Genetics Unit Tel: (0131) 3322471
Chromosome Biology Section ext: 2303 (office)
Western General Hospital 2311 (lab)
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