genomic DNA from plants?

Jose I. de las Heras Jose.delasHeras at hgu.mrc.ac.uk
Fri Feb 4 11:14:44 EST 2000


ZAG wrote:
> 
> Hi,
> 
> I´m working with A. thaliana and have to do a lot of DNA minipreps with
> just two leaves (about 50-100 mg) of material. Can anybody recommend me
> a cheap (i. e. not based on commercial columns) and reliable prep that
> produces DNA which can be digested?
> 
> thanks,
> 
> Harald F. Sigmund
> 
> hsigmund at edv2.boku.ac.at

Have you tried the standard CTAB-extraction method? I have used it
extensively for eucalypts and wheat. I usually extracted DNA from about
1-2g leaf tissue, to obtain a lot of DNA. But you can always scale it
down and modify it to make it quite fast too... Any questions email me.

Jose.

-- 
Jose I. de las Heras		Email: 	Jose.delasHeras at hgu.mrc.ac.uk
MRC Human Genetics Unit		Tel: 	(0131) 3322471
Chromosome Biology Section		ext: 	2303 (office)
Western General Hospital			2311 (lab)
Edinburgh EH4 2XU		Fax: 	(0131) 3432620	
Scotland - United Kingdom	

"Musha ring dum a doo dum a da, Whack for my daddy-o"




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