Ni affinity columns, preparation, use and recharging of

[Wolfgang Schechinger]

Hi all,

I'd like to learn ALL about Ni affinity columns.
Especially I'm looking for protocols, references and pointers to 
websites.

I'd like to know 
- how one may prepare colums (starting from agarose)
- the conditions for binding and elution of His6 tagged proteins
- how to clean and regenerate the resin
 
If someone who has some informations at hands could contribute them, 
this would be fine.

Thanks for all input, 

Wolfgang

Dr. Wolfgang Schechinger, Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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