Ni affinity columns, preparation, use and recharging of

Wolfgang Schechinger Wolfgang.Schechinger at
Sat Feb 5 13:26:55 EST 2000

Hi all,

I'd like to learn ALL about Ni affinity columns.
Especially I'm looking for protocols, references and pointers to 

I'd like to know 
- how one may prepare colums (starting from agarose)
- the conditions for binding and elution of His6 tagged proteins
- how to clean and regenerate the resin
If someone who has some informations at hands could contribute them, 
this would be fine.

Thanks for all input, 


Dr. Wolfgang Schechinger, Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wolfgang.schechinger at 
*unsolicited mail is *NOT* appreciated, usual disclaimers apply 
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