Immunoprecipitation and subsequent phosphotyrosine western

Neal Robert Melvin nrmelvin at
Sun Feb 6 00:54:17 EST 2000

Is it necessary to have orthovanadate present in my solutions after I've
transferred my proteins to my membrane?? For example, should it be present
in my blocking solution, and in with my primary antibody?

"Everyone tells me I'm lucky,
I've got my whole life to live yet,
I can't say they're wrong, 
but the days seem so long
Living inside of my head
Maybe I'll get some relief now,
Now that your things are all gone
I won't sit here staring at nothing all night
Bleary-eyed, greeting the dawn"

'Falling Down Blue'

Neal Melvin
University of Calgary - Faculty of Medicine
Department of Neuroscience - Neuroscience Research Group (NRG)
Health Sciences Centre
3330 Hospital Drive, NW
Calgary, Alberta, Canada
T2N 4N1

e-mail: nrmelvin at
Phone: (403) 220-4490 (lab)
       (403) 220-7035 (office)
       (403) 283-8731 (fax)

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