Ni affinity columns, preparation, use and recharging of

Arnoud van Vliet avvliet at knoware.nl
Sun Feb 6 16:11:03 EST 2000


Dear Wolfgang,

go to www.qiagen.com and get the QiaExpressionist Manual, or to the
www.invitrogen.com site and get their pTrcHis and Ni-agarose manual. Lots of
info and references to be found.

No affiliations with either.

hope this helps
Arnoud

"Wolfgang Schechinger" <Wolfgang.Schechinger at med.uni-tuebingen.de> wrote in
message news:200002051826.TAA21521 at intwww.zit.med.uni-tuebingen.de...
> Hi all,
>
> I'd like to learn ALL about Ni affinity columns.
> Especially I'm looking for protocols, references and pointers to
> websites.
>
> I'd like to know
> - how one may prepare colums (starting from agarose)
> - the conditions for binding and elution of His6 tagged proteins
> - how to clean and regenerate the resin
>
> If someone who has some informations at hands could contribute them,
> this would be fine.
>
> Thanks for all input,
>
> Wolfgang
>
> Dr. Wolfgang Schechinger, Pathobiochemistry Dept.
> University of Tuebingen, Germany
> email: wolfgang.schechinger at med.uni-tuebingen.de
> wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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