How to reduce the viscosity of Nuclear extractions??

[G. Dellaire, Ph.D.]

Hello,

Does anyone out there have a good method for reducing the viscosity of
nuclear extracts (mammalian) after high salt extraction?  I am having
trouble loading these extracts on SDS-PAGE gels as they are so bloody
viscous.  I have found that using a sonicator (barrel/tip) is not
practical if you have dozens of samples or if you have very small
volumes (under 100 ul to 30 uls typically).  The resulting foam eats up
most of your sample.

I have heard about using a sonicating water bath, has anyone used one of
these for this use?

any and all tips/replies are welcome.

Cheers,

Graham Dellaire

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Graham Dellaire, Ph.D.

MRC Human Genetics Unit
Western General Hospital
Edinburgh, UK
EH4 2XU
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