PCR and UV radiation

Roland Hübner rhubner at hgins.uia.ac.be
Mon Feb 7 09:03:01 EST 2000


In article <389EBC1A.AA429DB0 at pinnacle.jr2.ox.ac.uk>, 
jhughes at pinnacle.jr2.ox.ac.uk wrote:

> Hi does anybody know if exposing DNA to UV (i.e when cutting out a band
> on a transilluminator) in anyway effects the ability of taq polymerase

Hi Jim,

  depends on length of target, wavelength & time under UV... Also, what 
buffer and resuspension sol. (after possible purification) do you use? 
--> too much EDTA???  Think about dilution series etc.

Good luck,
 Roland




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