Radiolabel Mol. Wt. Marker

Colin Rasmussen colin at pombe.usask.ca
Mon Feb 7 10:06:34 EST 2000



Tracy Jo Litzi wrote:

> Hello everyone.  My question is: Can I radiolabel a molecular weight
> marker with P32 the same way I label my probes (random)?  I have
> labelled marker in the past but no longer remember if there was
> something special about the way I did it or if certain markers are
> better.  Thank you.

Nope.  The random priming make small randomly sized fragments, usually
around 2-300 bp in length.  If you want to labe markers, make them
end-labeled with T4 kinase.

Colin





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