How to reduce the viscosity of Nuclear extractions??

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Feb 7 11:14:05 EST 2000


In article <200002071548.IAA257642 at nestor.NMSU.Edu>, Hiranya S.
Roychowdhury <hroychow at nmsu.edu> writes
>Digest your samples with DNaseI. After the samples are prepared, add DNase
>to them (10ug/mL final conc.) and incubate at 37C for 30min.

Just make sure you get DNase that is protease free. Even at the level
above we can chew up pure proteins quite nicely.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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