How to reduce the viscosity of Nuclear extractions??
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Mon Feb 7 11:14:05 EST 2000
In article <200002071548.IAA257642 at nestor.NMSU.Edu>, Hiranya S.
Roychowdhury <hroychow at nmsu.edu> writes
>Digest your samples with DNaseI. After the samples are prepared, add DNase
>to them (10ug/mL final conc.) and incubate at 37C for 30min.
Just make sure you get DNase that is protease free. Even at the level
above we can chew up pure proteins quite nicely.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
More information about the Methods