How to reduce the viscosity of Nuclear extractions??

Hiranya S. Roychowdhury hroychow at nmsu.edu
Mon Feb 7 11:55:41 EST 2000


A very important point!

At 04:14 PM 2/7/00 +0000, Dr. Duncan Clark wrote:
>In article <200002071548.IAA257642 at nestor.NMSU.Edu>, Hiranya S.
>Roychowdhury <hroychow at nmsu.edu> writes
>>Digest your samples with DNaseI. After the samples are prepared, add DNase
>>to them (10ug/mL final conc.) and incubate at 37C for 30min.
>
>Just make sure you get DNase that is protease free. Even at the level
>above we can chew up pure proteins quite nicely.
>
>Duncan
>-- 
>The problem with being on the cutting edge is that you occasionally get 
>sliced from time to time....
>
>Duncan Clark
>DNAmp Ltd.
>Tel: +44(0)1252376288
>FAX: +44(0)8701640382
>http://www.dnamp.com
>http://www.genesys.demon.co.uk
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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