oligo cloning

Jered J Floyd jered at mit.edu
Mon Feb 7 15:49:01 EST 2000

Ooops, I failed to notice you were using a blunt-ended insert. That
makes things that much more difficult.  Make sure you are using lots
of extra ligase (I believe NEB recommends 50x for blunt-end fragments)
and ligate for 16 hours at 16C. If at all possible, see if you can
change your construct to be built with overhangs.


More information about the Methods mailing list