oligo cloning

Jered J Floyd jered at mit.edu
Mon Feb 7 15:49:01 EST 2000


Ooops, I failed to notice you were using a blunt-ended insert. That
makes things that much more difficult.  Make sure you are using lots
of extra ligase (I believe NEB recommends 50x for blunt-end fragments)
and ligate for 16 hours at 16C. If at all possible, see if you can
change your construct to be built with overhangs.

--jered




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