Unstable insert

panapat at netscape.net panapat at netscape.net
Mon Feb 7 21:05:56 EST 2000

 I have some problem or maintaining large insert in E.coli DH5 alpha.
The insert size about 10 kbs and vector is pUC18. I got clones that
contain this recombinant plasmid but the number is very low. Sometimes
there are some deletion in my gene. What should I do to maintain this
plasmid in E.coli? I need to get more DNA fro mutagenesis and protein
expression study.


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