Unstable insert

panapat at netscape.net panapat at netscape.net
Mon Feb 7 21:05:56 EST 2000


Hi,
 I have some problem or maintaining large insert in E.coli DH5 alpha.
The insert size about 10 kbs and vector is pUC18. I got clones that
contain this recombinant plasmid but the number is very low. Sometimes
there are some deletion in my gene. What should I do to maintain this
plasmid in E.coli? I need to get more DNA fro mutagenesis and protein
expression study.

Panapat


Sent via Deja.com http://www.deja.com/
Before you buy.




More information about the Methods mailing list