Mycoplasm help

[Dr. Fernando Rodriguez-Pascual]

Hi,

I am currently working with a human colon carcinoma cell line (DLD-1)
and in the last weeks I having problems with them and I suspect they can
have mycoplasm contamination. In particular, the cells do not grow,
many  are full of vacuoles and after a while most of them die. How can I
be sure that this is a mycoplasm contamination? Is there any easy method
to check it? If they are in fact contaminated, what kind of treatment
can I use to get rid of it? Among the problematic cells there are also
some stably transfected clones that I want to keep growing and healthy
(if possible)...

Thank you

Fernando
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Dr. Fernando Rodríguez Pascual
Department of Pharmacology
Johannes Gutenberg University
Mainz, Germany
email: frodrigu at mail.uni-mainz.de
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