oligo cloning

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Feb 8 05:04:12 EST 2000

In article <389F4115.878E178A at pombe.usask.ca>, Colin Rasmussen
<colin at pombe.usask.ca> writes
>What I would do is to use unkinased oligos, and leave the phosphate on the

By kinasing the oligos you could land up with multimers. 

I would also use unkinased oligos and leave the phosphate on the Vector
but use Xma I instead of Sma I. That way you leave a sticky overhang on
the vector. I would then redesign the oligos to have the same sticky
overhang in the appropriate reading frame etc.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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