a yeast two-hybrid system with C-terminal fusion proteins?

Tim Söllick soellick at mpiz-koeln.mpg.de
Tue Feb 8 06:20:58 EST 2000


Does anybody know about a yeast two-hybrid system that employs
C-terminal fusion of the GAL4 DNA binding domain and the GAL4 activation
domain (not as usual: N-terminal fusions of this domain to the protein
of your interest)?

Any references? Any vectors?

Any idea?







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